Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

In vitro study of drug-protein interaction using electronic absorption, fluorescence, and circular dichroism spectroscopy

In the near future, design of a new generation of drugs targeting proteins will be required. Considering the complex bond between the drug and protein, the structure and stability of the target protein should be considered. So far, a series of in vitro investigations have been conducted with the aim of predicting drug-biological medium interactions. In these studies, use of spectroscopic method...

Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy.

This article focuses on methods based on fluctuation correlation spectroscopy to determine the formation of protein complexes in living cells. We present the principles of the fluctuation method applied to cells. We discuss the novelty and the promises of this approach. The emphasis is in the discussion of the underlying statistical assumptions of the image correlation spectroscopy analysis rat...

Determination of critical micelle concentrations and aggregation numbers by fluorescence correlation spectroscopy: aggregation of a lipopolysaccharide.

Fluorescence correlation spectroscopy (FCS) is often used to determine the mass or radius of a particle by using the dependence of the diffusion coefficient on the mass and shape. In this article we discuss how the particle size of aggregates can be measured by using the concentration dependence of the amplitude of the autocorrelation function (ACF) instead of the temporal decay. We titrate a s...

Protein-protein interactions determined by fluorescence correlation spectroscopy.

Fluorescence correlation spectroscopy (FCS) is an emerging technique where the interaction between biomolecules is detected through their correlated motion. It offers the advantage of high (single-molecule) sensitivity; independence of molecular orientation or distance; and simultaneous measurement of molecular interactions, concentrations, and mobilities. Here we introduce the principle of the...

Fluorescence correlation spectroscopy.